Dr Тatjana Lj. Mitrović
Full Professor
Department of Biology with Ecology
Curriculum Vitae
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Name: TATJANA (Ljubiša) MITROVIĆ
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Contact e-mails: tatjanamitrović3@gmail.com; tatjana.mitrovic@pmf.edu.rs
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Address: Faculty of
Sciences and Mathematics, University of Niš, Višegradska 33, 18000 Niš, Serbia
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Web Page: https://npao.ni.ac.rs/prirodno-matematicki-fakultet/980-tatjana-mitrovic/980-tatjana-mitrovic; https://www.researchgate.net/profile/Tatjana-Mitrovic-3
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ORCID
ID: 0000-0001-5341-9831
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Scopus ID: 55382566800
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Google Scholar
ID: 9lBOibwAAAAJ
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Date and place
of birth: 28.07.1969., Niš, Serbia
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Citizenship: Serbian
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Research field
and areas: Glioma, Glioblastoma,
Biomarkers, Cancer Genomics & Transcriptomics, Non-coding RNAs, Precision
Medicine, Nanoparticles, Lichens, Plant extracts, Gene Therapy, Gene Regulation
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Education:
o 1988-1992
- B.Sc. in Molecular Biology and
Physiology, Faculty of Sciences,
Department of Biology, University of Belgrade,
Federal Republic of Yugoslavia
Title
of diploma work: “Subcloning and restriction mapping of DNA region upstream
from gene for gentamicine resistance Micromonospora
zionensis“
Field:
Molecular Biology
Diploma
work supervisor: Prof. dr Branka
Vasiljević, Full Professor in Molecular Genetics and Genetic Engineering of
Prokaryotes
Date of graduation: 29.12.1992.
o 1992-1998
- M.Sc. in Molecular Genetics and
Genetic Engineering, Faculty of Biology,
University of Belgrade, Federal
Republic of Yugoslavia
Title
of M.Sc. thesis: “Contribution of proximal and distal promoter of rat bbminy - globin
gene to its transcriptional activity“
Field:
Molecular Hematology; Molecular Genetics and Genetic Engineering
M.Sc.
thesis supervisor: Prof. dr Ana
Savić, Full Professor in Molecular Genetics and Genetic Engineering of
Eukaryotes
Date of graduation: 17.06.1998.
o 1998-2002
- Ph.D. in Molecular Genetics and
Genetic Engineering, Faculty of Biology,
University of Belgrade, Federal
Republic of Yugoslavia
Field:
Gene Therapy; Molecular Genetics and Genetic Engineering
Title
of Ph.D. thesis: “Selective amphotropic retroviral vectors for cancer gene
terapy“
Ph.D.
thesis supervisor: Prof. dr Bogomir
Dimitrijević, Full Professor in Molecular Biology of Cancer
Date of graduation: 17.07.2002.
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Dates of
appointments (researcher and scientific titles):
o Teaching Assistant in Biochemistry and Genetics (01.10.2001),
Department in Biology and Ecology, Faculty of Sciences and Mathematics,
University of Niš
o Assistant professor in Biotechnology (01.10.2003),
Department in Biology and Ecology, Faculty of Sciences and Mathematics,
University of Niš
o Associate
Professor in Biotechnology (01.10.2007), Department in Biology and Ecology,
Faculty of Sciences and Mathematics, University of Niš
o Full Professor in
Biotechnology (25.09.2012 - now), Department in Biology and Ecology, Faculty of
Sciences and Mathematics, University of Niš
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Employment
history:
o Research Fellow (June 1994 – June 1998) in Laboratory
for Molecular Hematology, Institute of Molecular Genetics and Genetic
Engineering (IMGGE), Belgrade, Federal Republic of Yugoslavia
o Research Assistant (June 1998 – 31.12.1999) in Laboratory for Molecular Hematology, IMGGE,
Belgrade, Federal Republic of Yugoslavia
o Post-doctoral Research
Fellow (25.06.2000 – 25.06.2001) in Laboratoire transfert génétique et
oncologie moléculaire, Institut d’hématologie, Hôpital Saint-Louis à
Paris, Université PARIS VII – Denis
Diderot and Laboratoire de transfert de gènes dans la
foie: applications thérapeutiques, Hôpital Antoine-Béclère à Clamart, Institut
Paris-Sud Cytokines, Université PARIS-SUD XI, France
o Teaching Assistant in Biochemistry and Genetics
(01.10.2001 - 01.10.2003), Department in Biology and Ecology, Faculty of
Sciences and Mathematics, University of Niš, Federal Republic of Yugoslavia
o Assistant professor in Biotechnology (01.10.2003-01.10.2007),
Department in Biology and Ecology, Faculty of Sciences and Mathematics,
University of Niš, Serbia and Montenegro
o Associate Professor in Biotechnology (01.10.2007-25.09.2012),
Department in Biology and Ecology, Faculty of Sciences and Mathematics,
University of Niš, Serbia
o Full Professor in Biotechnology (25.09.2012- now)
Department in Biology and Ecology, Faculty of Sciences and Mathematics,
University of Niš, Serbia
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Other:
o Member of Academic Council of the Faculty of Sciences
and Mathematics, University of Niš (2006-2008, 2008-2010, 2010-2013, 2013-2016,
2019-2021, 2021-now)
o Member of Electoral Council of the Faculty of Sciences
and Mathematics, University of Niš (2001-now)
o Member of Scientific Expert Council at the University
of Niš (2012-2015)
o Chair in Experimental Biology and Biotechnology (2018
– 2021)
o Head of the Laboratory for Molecular Biology, Biotechnology and Biomedicine (2013- now)
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Teaching:
Faculty
of Sciences and Mathematics, Niš:
o Bachelor of
Science in Biology and Bachelor of
Science in Ecology studies - list of courses: Genetics (2003-2007),
Biochemistry (2005-2007), Molecular Biology (2004-2007), Experimental
Biochemistry (2004-2007), Genotoxicology (2004-2007), Laboratory Practice
(2007-2008)
o Postgraduate Master of Science in Physiology and
Genetics studies (2004-2007) - list of courses: Advanced Molecular Biology,
Experimental Biochemistry, Genotoxicology
o Specialist
studies in Cellular and Molecular Biology (2004-2007) - list of courses: Advanced
Molecular Biology, Methods and Procedures in Cell and Molecular Biology,
Bioinformatics
o Аccredited
undergraduate studies Bachelor of
Science in Biology (2008-2021) - list of courses: Genetics, Molecular
Biology, Human Genetics
o Аccredited graduate studies Master of Science in Biology (2008-2021) - list of
courses: Basics of Genetic Engineering, Experimental Biochemistry
o Аccredited graduate studies Master of Science in Ecology (2008-2021) - list of
courses: Genotoxicology
o Аccredited postgraduate studies Doctor of Philosophy in
Biology (2013-2021) - list of courses: Genomics, Cancer Molecular Biology
o Аccredited postgraduate studies Doctor of Philosophy in
Computer Sciences (2013-2021) course Selected Chapters of Molecular Biology
o Newly accredited
undergraduate studies Bachelor of
Science in Biology (starting from October 1st 2021) list of
courses: Principles of Genetics, Molecular Biology
o Newly accredited graduate studies Master of Science in Biology (starting from October 1st
2021) Module Biology - list of courses: Experimental Biochemistry
o Newly accredited graduate studies Master of Science in Biology (starting from October 1st
2021) Module Molecular Biology and Physiology - list of courses: Molecular
Biology of Eukaryotes, Experimental Molecular Biology, Biotechnology, Basics of
Genetic Engineering and Biotechnology, Basics of Bioinformatics
o Newly accredited postgraduate
studies Doctor of Philosophy in Biology (starting from October 1st
2021) - list of courses: Genomics, Cancer Molecular Biology, Molecular Bases of
Diseases
o Newly accredited postgraduate
studies Doctor of Philosophy in Computer Sciences (starting from October 1st
2021) course Selected Chapters of Molecular Biology
University
of Niš:
o Аccredited graduate studies Master 4.0. - Intelligent
Data Analysis (2019-now) course Bioinformatics
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Project
history:
o
Molecular basis of transport through the cell membrane (1993-1994; grant
No. 1389 from Ministry of Science and
Technology of Republic Serbia; IMGGE, Belgrade, Federal Republic of Yugoslavia).
o
Molecular basis
of transport through the cell membrane -
Molecular hematology of hereditary anemias (1994-2000; grant No. 03E09
from Ministry of Science and
Technology of Republic Serbia IMGGE, Belgrade, Federal Republic of Yugoslavia).
o
Ciblage des vecteurs rétroviraux pour une
thérapie génique des tumeurs solides (carcinomes) (2000-2001; Laboratoire
transfert génétique et oncologie moléculaire, Institut d’hématologie, Hôpital
Saint-Louis à Paris, Université PARIS 7 Denis-Diderot,
France).
o
Thérapies cellulaire
et cellulaires des maladies métaboliques hépatiques (2001; Equipe Mixte
Inserm 00-20, Laboratoire de transfert de gènes dans la foie:
applications thérapeutiques, Hôpital Antoine-Béclère à Clamart, Institut
Paris-Sud Cytokines, Université PARIS-SUD XI,
France).
o
Transfert de gènes dans les
cellules tumorales (2001; Equipe Mixte Inserm 00-20, Laboratoire de transfert
de gènes dans la foie: applications thérapeutiques, Hôpital Antoine-Béclère à
Clamart, Institut Paris-Sud Cytokines, Université PARIS-SUD XI, France).
o
Clinical
implication of molecular heterogenety of
solid tumors (2002- 2004; grant No. 1691 from Ministry of Science, National Cancer Research Center, Belgrade,
Federal Republic of Yugoslavia).
o
Réponse
immunitaire antivirale systémique et cérébrale (2005; INSERM, Unité E109,
Laboratoire de réponse immunitaire antivirale systémique
et cérébrale, Faculté de Médecine Paris Sud à Le Kremlin Bicêtre, Institut Fédératif de
Recherche Bicêtre, Université PARIS-SUD XI, France).
o
Pharmacodinamical
and pharmacogenetical investigation of new drugs and predictive / prognostical
value of pharmacotherapy in oncology (2006 - 2010; grant No. 145035B from Ministry of Science
and Environmental Protection, National Cancer
Research Center, Belgrade, Serbia).
o
Regeneration of
bone tissue supported with biomaterials as tissue matrix - in vivo i in vitro study
(2006 - 2010; grant No. 145072 from
Ministry of Science and Environmental Protection, Faculty
of Medicine, Niš, Serbia).
o
Secondary
metabolites: composition, biological and antioxidant activity (2006 - 2010;
grant No.142054 from Ministry of Science and Environmental Protection, Faculty of Science and
Mathematics, Niš, Serbia).
o Molecular diagnostics of
glioblastoma (2016-2018; grant No. 36
from Faculty of Medicine, Niš, Serbia)
o Preventive, therapeutical and ethical approach to
preclinical and clinical research of genes and modulators of redox cellular signalization in immune,
inflammatory and proliferative cellular response (2010- now; grant No. III41018 from Ministry of Education,
Science and Technological Development, Faculty of
Medicine, Niš, Serbia).
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List of EU
projects:
o
Science teacher education
revision and upgrading (S.T.E.R.U.) (2006 – 2009; grant No. CD JEP 40053,
Tempus CARDS, national partners: University of Kragujevac, University of
Belgrade, University of Niš, University of Novi Sad, State University of Novi Pazar, Serbia; international
partners: University of Debrecen, Hungary and University of Jyvaskyla, Finland).
o
Higher education reform of biological sciences
(H.E.R.B.S.) (2006- 2009; grant No. CD JEP 40094, Tempus CARDS, national
partners: University of Belgrade, University of Kragujevac, University of Niš,
University of Novi Sad, State
University of Novi Pazar, Serbia; international partners: University of Nice -
Sophia-Antipolis, France, University of Torino, Italy and University of Madrid,
Spain).
o
Master program
for subject teachers in Serbia (M.A.S.T.S.) (2010-2014; grant No. 511170-TEMPUS-1-2010-1-RS-TEMPUS-JPCR
(2010-3197/001-001), contact person for the University of Niš, national partners: University of
Belgrade, University of Kragujevac, University of Niš, University of Novi Sad, State University of Novi
Pazar, Serbia; international partners: University of Innsbruck, Austria,
Metropolitan University College, Copenhagen, Danmark, University of Helsinky,
Finland and University of Debrecen, Hungary and University of Bath, UK).
o ICT Networking for Overcoming Technical and Social Barriers in Instrumental Analytical Chemistry Education (NETCHEM) (2016-2020; grant No. 573885-EPP-1-2016-1-RS-EPPKA2-CBHE-JP; Erasmus+ ; national partners: University of Niš, University of Belgrade, University of Kragujevac, University of Novi Sad, international partners: Agricultural University of Tirana, University of Tirana, Albania, Brno University of Technology, Brno, Czech republic, Université Pierre-et-Marie-Curie, Sorbonne Université, Paris, Le Commissariat à l’énergie atomique et aux énergies alternatives, Paris, France and University of Greenwich, London, UK).
o Glioma MR Imaging 2.0 (GliMR) (2019-2023; grant No. CA18206), European Cooperation in Science and Technology (COST)
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Awards,
prizes, fellowships:
o
1988. 1st place (gold medal) winner in Federal contest in
Biology, Federal Republic of Yugoslavia.
o
1989-1990. fellowship from University of Belgrade
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1990-1993. fellowship from Republic Foundation for the Development of
Scientific and Artistic Youth
o
1993-1994. fellowship from Ministry
of Science and Technology of Republic Serbia
o
2000-2001.
long-term fellowship La Ligue Nationale Contre le Cancer, France
o
2005. short-term fellowship from Ministry of Science and Enviromental Protection,
Serbia and Montenegro
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Reviewing of
the manuscripts for scientific journals: Current Research for Translational Medicine; Cancer; Life; Journal of Clinical Neuroscience; Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious
Diseases (MEEGID); Industrial
Crops and Products; Zeitschrift für Naturforschung
C; Biotechnology & Biotechnological Equipment; Radiology and Oncology; The
Cureus Journal of Medical Science; Agro FOOD Industry Hi-tech; Journal of the
Serbian Chemical Society; Facta Universitatis, Series: Medicine and Biology; Acta
Facultatis Medicae Naissensis.
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International
scientific collaboration and mobility:
o
June 2000 – June 2001. Laboratoire
transfert génétique et oncologie moléculaire, Institut
d’hématologie, Hôpital Saint-Louis à Paris, Université PARIS VII – Denis Diderot and Laboratoire
de transfert de gènes dans la foie: applications thérapeutiques, Hôpital
Antoine-Béclère à Clamart, Institut Paris-Sud Cytokines, Université PARIS-SUD
XI, France
o
June – September 2005. Laboratoire de réponse immunitaire antivirale systémique
et cérébrale, Institut Fédératif de Recherche Bicêtre à
Le Kremlin Bicêtre, Faculté de Médecine Paris Sud, Université
PARIS-SUD XI, France
o
November 2005. Département des Sciences de la Vie,
Université Nice-Sophia
Antipolis (Tempus CARDS project (JEP-40094-2005))
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Relevant
skills: DNA, RNA and protein
isolation; agarose and polyacrilamide gel electrophoresis; Southern, Northern
and Western Blot; Dot Blot; bisulfate conversion of DNA; PCR; RT-PCR and
Real-time PCR (qPCR); MSP; primer extension assay; plasmid DNA isolation; restriction
mapping; gene cloning; DNA sequencing; primary and secondary cell culture
techniques; transient and stable transformation of cell lines by
electroporation and calcium phosphate precipitation; CAT reporter gene
expression studies; β- GAL reporter gene assay; gel shift; design and
production of retroviral and lentiviral viral vectors for gene therapy;
transfection of retroviruses into packaging cell line and generation of stable
producer lines; preparation of virus stocks for gene therapy; determination of
virus titre; proliferation assay H3 – thymidine incorporation; TUNEL
assay for apoptosis
o
25th-28th October
1997 - EMBO workshop “Regulation and Function of GATA Proteins”, Capri, Italy
o
6th-8th June 2000 - EUROCANCER 2000, Paris, France
o
9th-11th June 2000 - 2nd
EUREGENETHY “Multidisciplinary Forum on Safety and Regulatory issues
in Gene Therapy”, Paris, France
o
14th-15th
November 2002 - XXXIX Cancer Week,
Serbian Medical Association, Beograd
o
12th-14th September 2002 - ESO
“State-of-the- art in prostate and breast cancer treatment”, 2002, Faculty of Medicine, Niš
o November 2003 -
XL Cancer Week, Serbian Medical Association, Beograd
o
16th – 17th October 2009 - 2nd
CPCTAS workshop “Pre-clinical testing of active
substances on cancer and various model systems”, Faculty
of Sciences and Mathematics, Kragujevac
o
13th-15th December 2010 - 4th CPCTAS
workshop “Antimicrobial activity of active substances”, Faculty
of Sciences and Mathematics, Niš
o
20th
November 2019 - “Labena” & “Bio-Rad” workshop “3 Generations of PCR”, Faculty of
Sciences and Mathematics, Niš
o 29th June 2020 “Qiagen”
webinar “QIAcuity
digital PCR”
o 10th November 2020 “Thermo Fisher Scientific” webinar “Innovative Western and Protein Analysis Solutions”
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Memberships:
o Serbian Genetic Society (from 1994 - now)
o Serbian Society for Molecular Biology (from 2015 -
now)
o Serbian Biological Society (from 2018 - now)
o European Hematology Association (1995 - 2005)
o European Society of Human Genetics (1998-2000)